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Evolutionary conservation and tissue-specific processing of Hoxa 11 antisense transcripts

S S Potter1, W W Branford

  • 1Division of Developmental Biology, Children's Hospital Medical Center and Department of Pediatrics, 3333 Burnet Ave., University of Cincinnati College of Medicine, Cincinnati, Ohio 45229-3039, USA.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|September 24, 1998
PubMed
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Antisense transcripts of the HOXA 11 gene are evolutionarily conserved across species, suggesting a regulatory role. These non-coding RNAs exhibit tissue-specific expression patterns and processing, highlighting their functional importance in development.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Previously identified abundant, processed, polyadenylated murine Hoxa 11 antisense transcripts.
  • Antisense transcripts in developing limbs showed complementary patterns to sense transcripts, suggesting regulatory function.
  • HOXA 11 gene's role in development and potential antisense regulation.

Purpose of the Study:

  • Analyze the human HOXA 11 genomic locus for conserved antisense transcript regions.
  • Identify and sequence human HOXA 11 fetal kidney antisense cDNAs.
  • Investigate evolutionary conservation and functional implications of HOXA 11 antisense transcription.

Main Methods:

  • Genomic locus analysis and cDNA sequencing.
  • Comparative analysis of human, mouse, and chicken HOXA 11 sequences.

Related Experiment Videos

  • Race in Circles technique to define transcription start sites.

    • Main Results:

    • Strong evolutionary conservation of human HOXA 11 antisense transcript regions.
    • Identification of polyadenylated human antisense RNAs with conserved 3' exon.
    • Tissue-specific differences in murine Hoxa 11 antisense transcription between limb and kidney.
    • Evidence against coding function for antisense transcripts based on mouse-human comparisons.

    Conclusions:

    • Evolutionary conservation of HOXA 11 antisense transcripts strongly suggests a functional role.
    • Antisense RNAs are processed differently and exhibit tissue-specific expression.
    • High selective pressure on HOXA 11 homeodomain sequences indicates functional specificity.