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Partial purification of hydrogenase from Anabaena sp. CH3 with heat treatment
Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology
|August 1, 1994
Abstract:
The methylviologen-dependent hydrogenase of Anabaena sp. CH3 is unstable at 4 degrees C and in air. All the purifying procedures were carried out at 25 degrees C and under argon atmosphere. The enzyme was partially purified by the following steps: heat treatment, DEAE-cellulose ion-exchange chromatography and gel filtration on TSK-Fractogel. Experimental results indicated that the heat-treatment procedure was beneficial for the separation of the enzyme from phycobiliproteins.