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Accessing lysozyme nucleation with a novel dynamic light scattering detector

R Peters1, Y Georgalis, W Saenger

  • 1ALV-Laser Vertiebsgesellschaft mbH, Robert-Bosch-Strasse 46, D-63225 Langen, Germany.

Acta Crystallographica. Section D, Biological Crystallography
|October 3, 1998
PubMed

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...