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RANTES augments eosinophil lucigenin-dependent chemiluminescence
J Chihara1, T Kakazu, I Higashimoto
1Department of Clinical and Laboratory Medicine, Akita University School of Medicine, Hondo, Akita, Japan.
International Archives of Allergy and Immunology
|October 6, 1998
Summary
Regulated on Activation, Normal T Expressed and Secreted (RANTES) enhances radical oxygen product release from eosinophils. This suggests RANTES plays a role in allergic inflammation by activating eosinophils.
Area of Science:
- Immunology
- Allergic Inflammation Research
Background:
- RANTES (regulated on activation, normal T expressed and secreted) exhibits chemotactic activity for eosinophils.
- Eosinophils are implicated in allergic inflammation via release of inflammatory molecules, including radical oxygen products.
Purpose of the Study:
- To investigate the effect of RANTES on radical oxygen product generation by eosinophils.
Main Methods:
- Eosinophils were isolated from asthma patients using CD16-negative depletion.
- Radical oxygen products were measured using lucigenin-dependent chemiluminescence.
- Eosinophils were stimulated with calcium ionophore A23187.
Main Results:
- RANTES treatment significantly increased both peak and integrated radical oxygen product values.
- Treated eosinophils showed higher chemiluminescence compared to untreated controls.
Conclusions:
- RANTES may contribute to allergic inflammation pathogenesis.
- RANTES enhances eosinophil oxidative metabolism, potentially increasing eosinophil activation and infiltration.