Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The DNA replication fork in eukaryotic cells

S Waga1, B Stillman

  • 1Cold Spring Harbor Laboratory, New York 11724, USA.

Annual Review of Biochemistry
|October 6, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolution of DNA replication origin specification and gene silencing mechanisms.

Nature communications·2020
Same author

Point prevalence survey for tick-borne pathogens in military working dogs, shelter animals, and pet populations in northern Colombia.

Journal of special operations medicine : a peer reviewed journal for SOF medical professionals·2014
Same author

Computer-based video analysis of movement.

The Australian journal of physiotherapy·2014
Same author

Perceptions of the clinical efficacy of laser therapy.

The Australian journal of physiotherapy·2014
Same author

Delayed endoscopic intraventricular hemorrhage (IVH) removal and endoscopic third ventriculostomy may not prevent consecutive communicating hydrocephalus if IVH removal was insufficient.

Minimally invasive neurosurgery : MIN·2007
Same author

Long-term mizoribine intermittent pulse therapy, but not azathioprine therapy, attenuated histologic progression in a patient with severe lupus nephritis.

Clinical nephrology·2007
Same journal

Lactate as a Chemical Modification on Proteins and Metabolites.

Annual review of biochemistry·2026
Same journal

Nucleocytoplasmic Transport.

Annual review of biochemistry·2026
Same journal

Packaging of Single-Stranded RNA in Viruses and Virus-Like Particles.

Annual review of biochemistry·2026
Same journal

Shaping of the Infant Gut Microbiome by Milk Oligosaccharides.

Annual review of biochemistry·2026
Same journal

Proteostasis Deregulation by Metabolism Drives the Hallmarks of Cancer.

Annual review of biochemistry·2026
Same journal

JoAnne Stubbe's Radical Path: A Story of Passion, Curiosity, and Persistence.

Annual review of biochemistry·2026
See all related articles

Eukaryotic DNA replication uses multiple proteins to accurately duplicate genomes. This coordinated process ensures cell cycle progression and DNA repair for genome integrity.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA replication is crucial for accurate genome duplication in eukaryotic cells.
  • Understanding replication fork progression is key to comprehending genome stability.

Purpose of the Study:

  • To elucidate the fundamental mechanisms of eukaryotic DNA replication fork progression.
  • To detail the protein machinery and processes involved in DNA synthesis and maturation.

Main Methods:

  • Biochemical studies using Simian Virus 40 origin of DNA replication in plasmid DNA.
  • Yeast genetic studies to investigate replication fork mechanisms.

Main Results:

  • Identified essential components including multiple DNA polymerases, single-stranded DNA-binding proteins, clamp-loading complexes, and polymerase clamps.

Related Experiment Videos

  • Described a DNA polymerase-switching mechanism for Okazaki fragment synthesis.
  • Highlighted the recruitment of nucleases, helicase, and ligase for Okazaki fragment maturation.

    • Conclusions:

    • Eukaryotic DNA replication is a highly coordinated process involving a complex interplay of proteins.
    • Replication is tightly coupled with cell-cycle progression and DNA repair pathways to maintain genome integrity.