Analysis of steroid hormone levels in female mice at high population density

Populations of predominantly female house mice (Mus musculus) were created by placing virgin female mice in cages (0.045 m2 to 0.48 m2) with a single stud male, and removing all ensuing male offspring at weaning. At maximum population size, the females in these all-female/one-male populations exhibited male-like aggressive behavior. Termination of the populations and subsequent measurement of steroid hormone levels indicated that the aggressive females had high circulating level of testosterone and corticosterone, and elevated baseline levels of progesterone. The high levels of corticosterone could be lowered by dexamethasone, but not the high levels of testosterone.