Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Crystallization of human complement component C5

R G Discipio1, L Jenner, S Thirup

  • 1La Jolla Institute for Experimental Medicine, 505 Coast Boulevard S., La Jolla, CA 92037, USA. richard@ljiem.org

Acta Crystallographica. Section D, Biological Crystallography
|October 8, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural and functional properties of the Kunitz-type and C-terminal domains of Amblyomin-X supporting its antitumor activity.

Frontiers in molecular biosciences·2023
Same author

Receptor-mediated exopolysaccharide perception controls bacterial infection.

Nature·2015
Same author

Crystallization and preliminary X-ray diffraction analysis of eukaryotic α2 -macroglobulin family members modified by methylamine, proteases and glycosidases.

Molecular oral microbiology·2014
Same author

Radial flow permeability testing of an argillaceous limestone.

Ground water·2012
Same author

Referral proformas improve compliance to national colorectal 2-week wait targets: does this affect cancer detection rates?

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2012
Same author

Isolation and crystallization of a chimeric Qβ replicase containing Thermus thermophilus EF-Ts.

Biochemistry. Biokhimiia·2010
Same journal

Structural insights into the synthesis of FMN in prokaryotic organisms.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

The first crystal structure of the peptidase domain of the U32 peptidase family.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.

Acta crystallographica. Section D, Biological crystallography·2015
See all related articles

Human complement component C5 (a key protein in the immune system) has been successfully crystallized. These stable, large crystals diffract X-rays to 3.3 A resolution, enabling structural studies.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Immunology

Background:

  • Human complement component C5 is a critical protein in the complement system, a part of innate immunity.
  • Understanding the structure of C5 is essential for developing targeted therapies for complement-mediated diseases.

Purpose of the Study:

  • To develop a method for crystallizing human complement component C5.
  • To characterize the resulting crystals and determine their diffraction properties.

Main Methods:

  • Low-salt batch crystallization technique.
  • X-ray diffraction analysis using rotating-anode and synchrotron radiation sources.
  • Cryoprotection using polyethylene glycol (PEG) 400.

Main Results:

Related Experiment Videos

  • Large hexagonal bi-pyramidal crystals of human complement component C5 were obtained.
  • Crystals exhibited remarkable stability in low salt and up to 2 M NaCl.
  • Diffraction resolution of 3.3 A was achieved using synchrotron radiation and cryoprotection.

Conclusions:

  • The developed crystallization method yields stable, high-quality crystals of human complement component C5.
  • These crystals are suitable for high-resolution structural determination.
  • The structural insights gained will advance the understanding of complement system function and disease.