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A volume adapter for use in a B-XIV zonal rotor
M Baggiolini1, B Dewald, U Bretz
1Research Institute Wander, Sandoz Research Unit, Wander Ltd., Berne, Switzerland.
Analytical Biochemistry
|November 1, 1978
Summary
A new adapter reduces the chamber volume of B-XIV rotors for analyzing small biological samples. This simple modification enhances rate-sedimentation analysis efficiency.
Area of Science:
- Biochemistry
- Molecular Biology
- Biophysical Chemistry
Background:
- Ultracentrifugation is crucial for separating biological molecules.
- B-XIV rotors are commonly used but have limitations with small sample volumes.
- Optimizing rotor chamber volume is key for sensitive analytical techniques.
Purpose of the Study:
- To describe a simple adapter for reducing the chamber volume of B-XIV-type rotors.
- To evaluate the adapter's suitability for rate-sedimentation analysis of small biological samples.
Main Methods:
- A disk-like adapter with an aluminum sleeve and four septa was designed.
- The adapter was fitted into the lower part of a B-XIV rotor chamber.
- The modified rotor's performance was assessed using rate-sedimentation analysis.
Main Results:
- The adapter successfully reduced the rotor chamber volume to 272 ml.
- The modified rotor proved highly suitable for analyzing small quantities of biological material.
- The modified rotor maintained standard B-XIV operational characteristics.
Conclusions:
- The described adapter offers a simple and effective solution for enhancing B-XIV rotor utility.
- This modification facilitates sensitive rate-sedimentation analysis with minimal biological material.
- The adapter integrates seamlessly with existing B-XIV rotor protocols.