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A simple method for synthesizing [gamma-32P]nucleoside triphosphates using [32P]acetylphosphate and acetate kinase
1Second Institute of Biochemistry, Semmelweis University, Medical School, Budapest, Hungary.
Analytical Biochemistry
|December 1, 1978
Abstract:
A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a final purification step. The entire procedure is performed 8 h or less.