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Isolation and sequence of cDNA encoding the motilin precursor from monkey intestine. Demonstration of the motilin
Z Huang1, P De Clercq, I Depoortere
1Gut Hormone Lab, University of Leuven, Belgium.
Abstract:
The motilin precursor cDNA has been isolated and sequenced from a cDNA library prepared from monkey small intestine. The sequence indicates a 345 bp open reading frame, a 63 bp 5' untranslated region and a 154 bp 3' untranslated region. The sequence encodes a 115 amino acid motilin precursor composed of a 25 amino acid signal peptide, the 22 amino acid motilin peptide and a 68 amino acid motilin associated peptide (MAP). Compared with the human motilin precursor cDNA, there are two amino acid substitutions in the signal peptide, one in motilin and four in the MAP. The presence of the motilin precursor in hypothalamus, hippocampus and cerebellum was demonstrated by RT-PCR.
Insights
Researchers isolated and sequenced monkey small intestine motilin precursor cDNA. This study details the motilin precursor
Area of Science:
- * Molecular Biology
- * Genetics
- * Neuroscience
Background:
- * Motilin is a gastrointestinal peptide hormone.
- * Understanding motilin precursor structure is crucial for gastrointestinal research.
Purpose of the Study:
- * To isolate and sequence the motilin precursor cDNA from monkey small intestine.
- * To analyze the structural differences between monkey and human motilin precursors.
- * To investigate the presence of the motilin precursor in the central nervous system.
Main Methods:
- * Construction and screening of a monkey small intestine cDNA library.
- * DNA sequencing to determine the open reading frame and untranslated regions.
- * Reverse Transcription Polymerase Chain Reaction (RT-PCR) to detect gene expression in brain tissues.
Main Results:
- * The monkey motilin precursor cDNA sequence was determined, revealing a 115 amino acid precursor.
- * Comparative analysis showed several amino acid substitutions between monkey and human motilin precursors.
- * RT-PCR confirmed the presence of the motilin precursor in the hypothalamus, hippocampus, and cerebellum.
Conclusions:
- * The monkey motilin precursor cDNA has been successfully isolated and characterized.
- * Significant sequence variations exist between monkey and human motilin precursors.
- * The motilin precursor is expressed in key brain regions, suggesting potential roles beyond the gastrointestinal tract.