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Antiviral effect of proline-rich polypeptide in murine resident peritoneal cells
B Orzechowska1, M Janusz, B Domaraczenko
1Laboratory of Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Abstract:
It is known that resident peritoneal (RP) cells from BALB/c female mice express a constitutive non-specific antiviral immunity which is progressively reduced during several days of cultivation in vitro. In this report, we have studied the effect of a proline-rich polypeptide (PRP) isolated from ovine colostrum on the kinetics of vesicular stomatitis virus (VSV) replication in freshly isolated and one-day cultured RP cells. The polypeptide was added to the cells immediately after virus adsorption or one day before or after viral infection. Independently on time of PRP addition, an inhibition of VSV replication (virus titres reduced by up to 4 log units) was observed. Occasionally, however, a weak stimulation of VSV replication by PRP (virus titres increased by 1-2 log units) was noticed in RP cells constitutively resistant to the infection.
Insights
Ovine colostrum proline-rich polypeptide (PRP) inhibits vesicular stomatitis virus (VSV) replication in mouse peritoneal cells. This antiviral effect was observed regardless of when PRP was administered relative to viral infection.
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- Resident peritoneal (RP) cells from BALB/c female mice possess inherent antiviral immunity.
- This constitutive antiviral defense diminishes over time during in vitro cell culture.
Purpose of the Study:
- To investigate the impact of ovine colostrum-derived proline-rich polypeptide (PRP) on vesicular stomatitis virus (VSV) replication.
- To determine how PRP affects VSV replication kinetics in both freshly isolated and cultured RP cells.
Main Methods:
- PRP was administered to RP cells at different time points: immediately after VSV adsorption, one day before infection, or one day after infection.
- VSV replication was quantified by measuring virus titers in treated and untreated RP cells.
Main Results:
- PRP significantly inhibited VSV replication, reducing virus titers by up to 4 log units.
- This inhibitory effect was consistent irrespective of the timing of PRP addition.
- A minor stimulation of VSV replication was occasionally observed in RP cells that were constitutively resistant to VSV infection.
Conclusions:
- Ovine colostrum PRP exhibits potent antiviral activity against VSV in mouse peritoneal cells.
- PRP demonstrates efficacy in modulating viral replication kinetics, suggesting therapeutic potential.