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Colorimetric determination of piperazine
Indian Journal of Physiology and Pharmacology
|July 1, 1976
Summary
A new, precise method accurately quantifies piperazine using a reineckate complex. This colorimetric technique is effective for biological samples and sensitive across a wide range of concentrations.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Accurate quantification of piperazine is crucial in various scientific fields.
- Existing methods may lack sensitivity or applicability to biological matrices.
Purpose of the Study:
- To develop and describe a sensitive and precise quantitative method for piperazine.
- To validate the method's linear response range and applicability to biological samples.
Main Methods:
- Formation of a piperazine-reineckate complex in neutral or acidic conditions.
- Separation of the complex via centrifugation.
- Dissolution in acetone and colorimetric estimation at 530 nm.
Main Results:
- The method demonstrates high sensitivity and precision.
- Linear response observed from 25 µg to 500 µg and above.
- Successful application to piperazine determination in trichloroacetic acid extracts of biological samples.
Conclusions:
- The described colorimetric method offers a reliable approach for piperazine quantification.
- Its sensitivity and applicability to biological samples make it a valuable analytical tool.