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[A recombinant autoantigen U 1 RNP 70,000: expression in E. coli. and its serodiagnostic application]
1Department of Rheumatology, Renji Hospital, Shanghai Second Medical University, Shanghai Immunology Institute.
Zhonghua Yi Xue Za Zhi
|May 1, 1997
Objective:
To express the recombinant autoantigen U 1 RNP 70,000 and to evaluate its serodiagnostic value.
Method:
We used molecular cloning techniques and immunoblotting technique.
Results:
The recombinant proteins had authentic U 1 RNP 70,000 antigenicity, with the molecular weight corresponding to their theoretical value. 112 of 115(97.4%) anti-70,000 positive sera(IBT) could be detected by r 70,000.
Conclusions:
r 70,000 has good specificity and can be used in further detailed epitopes mapping. r 70,000 can be put into use for serodiagnostic detection.