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Standardizing flow cytometry: a classification system of fluorescence standards used for flow cytometry
A Schwartz1, G E Marti, R Poon
1Flow Cytometry Standards Corporation, San Juan, Puerto Rico, USA. fcsc@caribe.net
Cytometry
|October 17, 1998
Summary
Selecting the right fluorescence standards for flow cytometry quality assurance is simplified by a new classification system based on physical characteristics. This system aids in choosing appropriate standards for alignment, calibration, and interlaboratory comparisons, improving research and clinical applications.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Immunology
Background:
- The proliferation of fluorescence standards in flow cytometry complicates quality assurance.
- Users face challenges in selecting appropriate standards for specific applications.
Purpose of the Study:
- To develop a classification system for fluorescence standards based on physical characteristics.
- To guide flow cytometer users in selecting standards for optimal quality assurance.
Main Methods:
- A classification system for fluorescence standards was developed.
- Standards were categorized based on their physical properties.
- The functional capabilities of standards for alignment, referencing, compensation, and calibration were assessed.
Main Results:
- Physical characteristics dictate the performance of fluorescence standards in key functions.
- The classification system links standard properties to specific applications like intra- and interlaboratory quality assurance.
- Standard classification facilitates unified analysis across different instruments and laboratories.
Conclusions:
- Understanding the classification system enhances instrument setup and quality control in flow cytometry.
- This system supports efficient and accurate research and clinical applications by enabling appropriate standard selection.
- It promotes independent and direct comparison of instrument performance, crucial for multi-laboratory studies.