Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitation of CD38 expression using QuantiBRITE beads

S B Iyer1, L E Hultin, J A Zawadzki

  • 1Becton Dickinson Immunocytometry Systems, San Jose, California, USA. sujata_iyer@bdis.com

Cytometry
|October 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep Learning Segmentation of the Nucleus Basalis of Meynert on 3T MRI.

AJNR. American journal of neuroradiology·2023
Same author

Widespread theta synchrony and high-frequency desynchronization underlies enhanced cognition.

Nature communications·2017
Same author

Preferential depletion of gut CD4-expressing iNKT cells contributes to systemic immune activation in HIV-1 infection.

Mucosal immunology·2012
Same author

Non-operative management of acute cholecystitis in the elderly.

The British journal of surgery·2012
Same author

Gastroduodenal perforation: maximal or minimal intervention?

Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society·2010
Same author

Influence of maternal corticosterone treatment on incubation length of eggs laid by Japanese quail hens selected for divergent adrenocortical stress responsiveness.

British poultry science·2009

The QuantiBRITE bead method reliably quantifies CD38 expression on CD8+ T-lymphocytes. This method, using specific repurified antibodies, aligns with the CD4 biological calibration method for accurate results in HIV research.

Area of Science:

  • Immunology
  • Virology
  • Biotechnology

Background:

  • CD38 is a marker on CD8+ T-lymphocytes, crucial for understanding HIV pathogenesis.
  • Accurate quantitation of CD38 expression is essential for prognostic interpretation.

Purpose of the Study:

  • To compare the QuantiBRITE bead method with the CD4 biological calibration method for quantifying CD38 expression.
  • To establish conditions for consistent and reliable CD38 quantitation.

Main Methods:

  • Comparison of QuantiBRITE and CD4 biological calibration methods.
  • Use of repurified CD38-phycoerythrin (PE) and CD4-PE conjugates.
  • Analysis of CD38 expression on CD8+ T-lymphocytes from Multicenter AIDS Cohort Study participants.

Main Results:

Related Experiment Videos

  • The QuantiBRITE method yielded identical results to the CD4 biological calibration method under specific conditions.
  • Repurified CD38-PE and CD4-PE are critical for accurate quantitation.
  • Standardized sample preparation methods (NH4Cl, FACS lysing, buffer dilution, paraformaldehyde fixation) showed consistent CD38 relative fluorescence intensity.

Conclusions:

  • Repurified PE conjugates of CD38 and QuantiBRITE PE beads offer a convenient and reliable method for CD38 quantitation.
  • Prognostic significance of QuantiBRITE-derived CD38 values can be interpreted using established reports.
  • Understanding antibody valence is important for antigen density determination from antibodies bound per cell (ABCs).