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AbeI, a restriction endonuclease from Azotobacter beijerinckii, which recognizes the asymmetric heptanucleotide
J Vitkute1, Z Maneliene, A Janulaitis
1Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.
Nucleic Acids Research
|October 20, 1998
Abstract:
A new restriction endonuclease Abe I has beenisolated from Azotobacter beijerinckii. This enzymerecognizes the asymmetric heptanucleotide sequence 5'-CCTCAGC-3' and cleaves within it symmetrically at positions -5/-2 in the opposing strands, producing three base protruding 5'-ends.