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Related Experiment Videos

Swelling-induced decrease in spontaneous pacemaker activity of rabbit isolated sino-atrial node cells

M Lei1, P Kohl

  • 1University Laboratory of Physiology, Oxford, UK.

Acta Physiologica Scandinavica
|October 20, 1998
PubMed
Summary
This summary is machine-generated.

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Sino-atrial node cells slow their heart rate when swelling, contrary to expectations. This study reveals that cell swelling reduces pacemaker rate through cytosolic dilution and decreased potassium currents, not volume-activated channels.

Area of Science:

  • Cardiovascular Physiology
  • Cellular Electrophysiology

Background:

  • The heart rate increases with sino-atrial node wall stress.
  • Swelling-activated ion channels are hypothesized to mediate this response.

Purpose of the Study:

  • To investigate the direct effects of cell swelling on rabbit sino-atrial node pacemaker cell activity.
  • To determine the mechanisms underlying the cellular response to swelling.

Main Methods:

  • Current clamp and voltage clamp electrophysiology on isolated rabbit sino-atrial node cells.
  • Amphotericin-permeabilized patch recordings.
  • Computer modeling (OXSOFT Heart v4.8) for mechanistic analysis.

Main Results:

  • Cell swelling (75% hyposmotic) decreased spontaneous pacemaker rate by 24.2% (P < 0.01).

Related Experiment Videos

  • Swelling caused a positive shift in the IK reversal potential (5-10 mV) and reduced IKr amplitude.
  • Computer modeling implicated cytosolic dilution and reduced IK as key mechanisms.

    • Conclusions:

    • Spontaneously active sino-atrial node cells reduce pacemaking rate during swelling.
    • This response is not mediated by known volume-activated channels but by other factors like cytosolic dilution and reduced IK.
    • Cardiac responses to cell volume changes differ from those to mechanical stretch.