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A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome
J Herold1, V Thiel, S G Siddell
1Institute for Virology and Immunology, University of Würzburg, Germany.
Advances in Experimental Medicine and Biology
|October 23, 1998
Abstract:
A strategy to generate in vitro transcripts representing infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome is presented. PCR-DNAs were ligated in vitro, resulting in 27 kbp and 22 kbp ligation products. These DNAs can now be transcribed in vitro and the RNAs tested for infectivity and their ability to replicate.