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Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
Published on: December 13, 2016
Biomedical applications of maghemite ferrofluid
A Halbreich1, J Roger, J N Pons
1Laboratoire des Milieux Désordonnés et Hétérogènes, Université Pierre-et-Marie-Curie, Paris, France.
Biochimie
|October 23, 1998
Summary
Cell-targeted ferrofluid (FF) detects phosphatidylserine (PS) exposure on red blood cells (RBCs), aiding in early disease diagnosis. This method shows promise for identifying malaria, sickle cell anemia, and Alzheimer
Area of Science:
- Biotechnology
- Nanotechnology
- Cell Biology
Background:
- Cell membrane modifications are crucial indicators of various diseases.
- Early detection of phosphatidylserine (PS) exposition on the outer leaflet of the plasma membrane is vital for understanding cellular health.
- Existing methods for detecting PS exposition have limitations in sensitivity and early detection.
Purpose of the Study:
- To review the application of cell-targeted ferrofluid (FF) in characterizing cell membrane modifications.
- To highlight the sensitivity of FF-bound recombinant human annexin V (AnxFF) in detecting early signs of PS exposition.
- To explore the diagnostic potential of AnxFF in various diseases and conditions.
Main Methods:
- Synthesis of Maghemite ferrofluid (FF) using the Massart method, complexed with dimercaptosuccinic acid.
- Development of cell targeting by coupling FF to biological effectors like antibodies and lectins for magnetic cell sorting.
- Utilizing FF bound to recombinant human annexin V (AnxFF) for sensitive detection of PS exposition on red blood cells (RBCs).
- Combining AnxFF binding with cell electrophoretic mobility, glycerol resistance, and filterability tests to characterize RBC membrane modifications.
Main Results:
- AnxFF detected PS exposition on mouse RBCs after 24-h storage and transiently after Plasmodium infection.
- AnxFF identified PS exposition on RBCs from sickle cell anemia patients and after 20 days of blood storage.
- AnxFF-based analysis, combined with other tests, suggested continuous damage and regeneration in RBCs of Alzheimer's disease patients, with potential for 95% accurate diagnosis.
- Magnetocytolysis was achieved using FF on THP1 cells and macrophages without damaging non-bound cells or heating the solution.
Conclusions:
- Cell-targeted ferrofluid, particularly AnxFF, offers a highly sensitive method for early detection of PS exposition on RBCs.
- This technique has significant diagnostic potential for infectious diseases (malaria), hematological disorders (sickle cell anemia), and neurodegenerative diseases (Alzheimer's).
- Ferrofluid-based magnetocytolysis presents a targeted cell destruction method with potential therapeutic applications.
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