Related Experiment Video
Updated: Aug 8, 2026
Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation
Published on: March 14, 2017
Regulation of parathyroid hormone-related protein expression in a canine squamous carcinoma cell line by colchicine
A Gröne1, M T Weckmann, C C Capen
1Department of Veterinary Biosciences, The Ohio State University, Columbus, USA.
Abstract:
The regulation of parathyroid hormone-related protein expression by colchicine, vinblastine, nocodazole, taxol, transforming growth factor-beta1 (TGFbeta1), and epidermal growth factor (EGF) was investigated in a canine squamous carcinoma cell line (SCC 2/88 cells). SCC 2/88 cells were stably transfected with a human P2/P3 PTHrP promoter-luciferase reporter gene construct and gene expression was measured after chemical treatments. The greatest increase in reporter gene expression was observed after colchicine treatment and small increases occurred after treatment with vinblastine, taxol, TGFbeta1, or EGF. Nocodazole had no significant effect on reporter gene expression. Colchicine also increased PTHrP steady state mRNA expression and PTHrP secretion by SCC 2/88 cells. These results demonstrated that PTHrP production was increased in SCC 2/88 cells by colchicine and suggested that factors or events during mitosis are capable of stimulating PTHrP production. An increase in PTHrP production during mitosis of malignant epithelial cells may be important in the pathogenesis of humoral hypercalcemia of malignancy.
Insights
Colchicine treatment significantly increased parathyroid hormone-related protein (PTHrP) expression in cancer cells. This suggests that events during mitosis can stimulate PTHrP production, potentially contributing to hypercalcemia of malignancy.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Parathyroid hormone-related protein (PTHrP) plays a role in humoral hypercalcemia of malignancy.
- Understanding the regulation of PTHrP expression is crucial for cancer research.
Purpose of the Study:
- To investigate the regulation of PTHrP expression in canine squamous carcinoma cells (SCC 2/88).
- To determine the effect of specific agents, including microtubule-disrupting drugs and growth factors, on PTHrP production.
Main Methods:
- SCC 2/88 cells were transfected with a PTHrP promoter-luciferase reporter gene construct.
- Gene expression was measured following treatment with colchicine, vinblastine, nocodazole, taxol, TGFbeta1, and EGF.
- PTHrP mRNA levels and secretion were assessed.
Main Results:
- Colchicine treatment caused the greatest increase in reporter gene expression.
- Vinblastine, taxol, TGFbeta1, and EGF showed smaller increases in gene expression.
- Nocodazole had no significant effect.
- Colchicine also increased PTHrP mRNA and secretion.
Conclusions:
- PTHrP production is stimulated by colchicine in SCC 2/88 cells.
- Factors or events occurring during mitosis can enhance PTHrP production.
- Increased PTHrP during mitosis of malignant epithelial cells may contribute to the pathogenesis of humoral hypercalcemia of malignancy.
Related Concept Videos
02:34Inhibition of Cdk Activity
01:17Hormones and Bone Tissue
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...