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Intrinsic and extrinsic NPY nerves in transplanted neuroinsular complexes
S Persson-Sjögren1, S Forsgren, U Kjörell
1Department of Histology and Cell Biology, Umeå University, Sweden. Solveig.Persson-Sjogren@histocel.umu.se
Abstract:
In mouse pancreatic islets, whether in situ or transplanted to kidney, nerve fibers and a few perikarya expressed NPY-like immunoreactivity (NPY-LI). In 4-5 day old grafts, NPY-LI coexisted with VIP-LI in randomly distributed nerve fibers. By 2-52 weeks, NPY mainly co-existed with tyrosine hydroxylase in fibers emanating from the kidney parenchyma. Radioimmunoassays indicated that the NPY levels increased with time, while those of VIP decreased. The study shows that NPY is primarily present in the intrinsic VIP-ergic innervation of islet grafts but later is mainly a constituent of the ingrowing sympathetic innervation.