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Related Experiment Videos

Secretable human platelet-derived factor V originates from the plasma pool

R M Camire1, E S Pollak, K Kaushansky

  • 1Department of Biochemistry, University of Vermont, College of Medicine, Burlington; the Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Blood
|October 27, 1998
PubMed
Summary

Platelets primarily obtain factor V (FV) by megakaryocytes endocytosing it from plasma, not solely synthesizing it. This finding clarifies the origin of platelet-derived FV and its role in blood coagulation.

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Area of Science:

  • Hematology
  • Molecular Biology
  • Biochemistry

Background:

  • Factor V (FV) is crucial for the prothrombinase complex, acting as a cofactor.
  • The origin of platelet-derived FV has been debated, with megakaryocytes considered a primary source.
  • FVLeiden is a common mutation affecting FV function and thrombosis risk.

Observation:

  • Two patients heterozygous for FVLeiden underwent transplantation, allowing investigation of FV origin.
  • Analysis of platelet-derived FV in these patients revealed a mixed or wild-type phenotype.
  • Experiments showed no significant uptake of radiolabeled FV by platelets, refuting direct endocytosis.

Findings:

  • Secretable platelet-derived FV originates mainly from endocytosis by megakaryocytes from plasma.

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  • This process is independent of the megakaryocyte's own genetic FV status (e.g., FVLeiden).
  • Platelets do not directly endocytose plasma-derived FV.

    • Implications:

    • Establishes a novel understanding of FV sourcing in platelets, impacting coagulation research.
    • Provides insights into FV metabolism and its potential role in thrombotic disorders.
    • Clarifies the contribution of plasma vs. intrinsic synthesis to platelet FV content.