Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The second stalk: the delta-b subunit connection in ECF1F0

I Ogilvie1, S Wilkens, A J Rodgers

  • 1Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA.

Acta Physiologica Scandinavica. Supplementum
|October 28, 1998
PubMed
Summary

The ATP synthase F1F0, a tiny molecular motor, uses ATP hydrolysis to power rotation. New evidence suggests the delta and b subunits form a secondary stalk, acting as a stator to aid these movements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of lablab (Lablab purpureus (L.) Sweet) for bio-control by native arthropods and its effect on yield of pumpkins.

Bulletin of entomological research·2015
Same author

[1st diagnosis of a true "hepar mobilis" in childhood].

RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin·2011
Same author

Cytochrome C oxidase-lipid interface from the protein side.

Biophysical journal·2009
Same author

Isolated deficiencies of OXPHOS complexes I and IV are identified accurately and quickly by simple enzyme activity immunocapture assays.

Biochimica et biophysica acta·2008
Same author

Mitochondrial dysfunction in focal segmental glomerulosclerosis of puromycin aminonucleoside nephrosis.

Kidney international·2006
Same author

Antibody-based approaches to diagnosis and characterization of oxidative phosphorylation diseases.

Mitochondrion·2005

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biophysics

Background:

  • ATP synthase (F1F0) is the smallest known molecular motor.
  • Its function involves ATP hydrolysis driving rotary motion.
  • Understanding its mechanical coupling is crucial.

Purpose of the Study:

  • To review evidence on the structural and functional roles of ATP synthase subunits.
  • To elucidate the mechanism of rotary motion facilitation.
  • To highlight the role of the delta and b subunits.

Main Methods:

  • Literature review of existing experimental data.
  • Analysis of structural and biochemical studies on ATP synthase.
  • Integration of evidence regarding subunit interactions.

Related Experiment Videos

Main Results:

  • ATP hydrolysis drives the rotation of gamma and epsilon subunits within the F1 sector.
  • The alpha3 beta3 complex forms the stable part of F1.
  • The delta and b subunits constitute a second stalk.
  • This second stalk functions as a stator, stabilizing rotational movements.

Conclusions:

  • The delta and b subunits are essential for stator function in ATP synthase.
  • This stator mechanism facilitates the rotary motion driven by ATP hydrolysis.
  • The F1F0 complex represents a sophisticated molecular machine with distinct functional components.