Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Measuring [Ca2+] with fluorescent indicators: theoretical approach to the ratio method

P A Gomes1, R A Bassani, J W Bassani

  • 1Department of Biomedical Engineering, Faculty of Electrical Engineering and Computing, Universidade Estadual de Campinas, SP, Brazil.

Cell Calcium
|October 30, 1998
PubMed
Summary

This study analyzes the ratio method for measuring intracellular calcium concentration ([Ca2+]i). We determined the method

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design of a Multipoint Cost-Effective Optical Instrument for Continuous In-Situ Monitoring of Turbidity and Sediment.

Sensors (Basel, Switzerland)·2020
Same author

Ecosystem Services Provided by Insects in Brazil: What Do We Really Know?

Neotropical entomology·2020
Same author

Development of a Cost-Effective Optical Sensor for Continuous Monitoring of Turbidity and Suspended Particulate Matter in Marine Environment.

Sensors (Basel, Switzerland)·2019
Same author

Functional antagonism of β-adrenoceptor subtypes in the catecholamine-induced automatism in rat myocardium.

British journal of pharmacology·2010
Same author

Transient outward potassium current and Ca2+ homeostasis in the heart: beyond the action potential.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2006
Same author

Inhibition of the sarcoplasmic reticulum Ca2+ pump with thapsigargin to estimate the contribution of Na+-Ca2+ exchange to ventricular myocyte relaxation.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2003

Area of Science:

  • Biophysics
  • Cell Biology
  • Analytical Chemistry

Background:

  • The ratio method is crucial for quantifying intracellular calcium concentration ([Ca2+]i).
  • Accurate calibration is essential for reliable [Ca2+]i measurements using fluorescence-based techniques.
  • Understanding the physical basis of fluorescence measurements enhances method interpretation.

Purpose of the Study:

  • To provide a theoretical analysis of the ratio method for intracellular calcium concentration measurement.
  • To determine the sensitivity and error propagation of the ratio method.
  • To offer a microscopic interpretation of fluorescence phenomena and calibration parameters.

Main Methods:

  • Theoretical analysis of the ratio method equation for [Ca2+]i estimation.

Related Experiment Videos

  • Analysis of error propagation related to calibration parameter inaccuracies.
  • Development and proposal of a new approach for accurate estimation of the beta parameter.

    • Main Results:

    • The maximum sensitivity of the ratio method is identified at specific [Ca2+]i conditions.
    • Inaccuracies in calibration parameters significantly impact measurement errors.
    • The beta parameter is shown to be dependent on both experimental setup and indicator spectrum.
    • A novel method for estimating beta yielded statistically comparable results to traditional methods.

    Conclusions:

    • The theoretical framework clarifies the ratio method's performance and limitations for [Ca2+]i measurement.
    • Accurate calibration and understanding of the beta parameter are critical for precise intracellular calcium quantification.
    • The proposed approach offers a more accurate estimation of beta, improving the reliability of the ratio method.