Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio)

M Nakao1, Y Fushitani, K Fujiki

  • 1Laboratory of Marine Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.

Journal of Immunology (Baltimore, Md. : 1950)
|October 30, 1998
PubMed
Summary

Researchers identified two novel complement factor B/C2-like genes in carp. One gene, B/C2-A, is similar to known vertebrate factor B and C2, while B/C2-B has unique features and likely doesn't function as a C3 convertase.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detrimental effects of Notch1 signaling activated by cadmium in renal proximal tubular epithelial cells.

Cell death & disease·2014
Same author

The influence of mechanical stimulation on osteoclast localization in the mouse maxilla: bone histomorphometry and finite element analysis.

Biomechanics and modeling in mechanobiology·2012
Same author

The sex determining loci and sex chromosomes in the family salmonidae.

Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation·2009
Same author

Rainbow trout interleukin-2: cloning, expression and bioactivity analysis.

Fish & shellfish immunology·2009
Same author

Induction of mononuclear cell infiltration into liver by Japanese herbal medicine.

Drugs under experimental and clinical research·2006
Same author

Methotrexate-induced acute lung injury in a patient with rheumatoid arthritis.

International journal of clinical pharmacology research·2005

Area of Science:

  • Immunology
  • Evolutionary Biology
  • Genetics

Background:

  • Mammalian complement system involves factor B and C2 in C3 convertase formation.
  • These components are thought to have arisen from a gene duplication event.
  • The timing of this B/C2 duplication remains unclear.

Purpose of the Study:

  • To investigate the evolutionary origins of complement factor B and C2.
  • To identify and characterize B/C2-like genes in a bony fish model, the common carp (Cyprinus carpio).
  • To understand the structural and potential functional divergence of these genes.

Main Methods:

  • Isolation and sequencing of B/C2-like cDNA clones from carp.
  • Phylogenetic analysis to determine evolutionary relationships.

Related Experiment Videos

  • Northern and Southern blotting to assess gene expression and copy number.

    • Main Results:

    • Two diverged B/C2-like cDNA clones, B/C2-A and B/C2-B, were identified in carp.
    • B/C2-A exhibits conserved domain structure similar to known vertebrate factor B and C2.
    • B/C2-B possesses unique structural features and a substitution rendering it unlikely to function as a C3 convertase.
    • Northern blotting showed distinct transcripts for both B/C2-A and B/C2-B.
    • Southern blotting indicated multiple genes for B/C2-A and a single gene for B/C2-B.

    Conclusions:

    • Carp possess diverged B/C2-like genes, offering insights into complement system evolution.
    • B/C2-A represents a more conserved form, while B/C2-B shows significant divergence.
    • The findings contribute to understanding the ancient gene duplication event predating mammalian divergence.