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Determination of provitamin A of green leafy vegetables by high performance liquid chromatography and open column
L B de Almeida-Muradian1, M D Rios, R Sasaki
1Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, Brasil. ligiabi@usp.br
Abstract:
The purpose of study was to determine the provitamin A value through beta-carotene analysis of five Brazilian leafy vegetables: carrot leaves (Daucus carota L.), beet leaves (Beta vulgaris L.) "serralha" (Sonchus oleracea L.), mint (Mentha piperita LL.) and celery leaves (Aplum graveolens. L.). Two analysis methods were used: open-column chromatography (OCC) and high performance liquid chromatography (HPLC). Two analysis methods were used: open-column chromatography (OCC) and high performance liquid chromatography (HPLC). Recovery tests were done for both methods and 92% of recovery was obtained for OCC and for HPLC 102%. The provitamin A value obtained for these leaves, using OCC were: 491 RE/100 g for carrot, 1097 for beet, 1390 for "serralha", 1016 for mint and 911 for celery. The results obtained using HPLC were 495 RE/100 g for carrot leaves, 1095 for beet leaves, 1472 for "serralha" leaves, 940 for mint leaves and 908 for celery leaves. We concluded that both methods presented good recoveries, are similar for determination of provitamin A in the leaves and they can be used for routine analysis. These five leaves can be as an inexpensive and easily obtained sources of provitamin A in Brazil.