Pancreatoblastoma: optically clear nuclei in squamoid corpuscles are rich in biotin

Pancreatoblastoma is a rare pancreatic neoplasm that generally occurs in children and demonstrates unique histopathologic features, including squamoid corpuscles. We encountered five cases of pancreatoblastoma and unexpectedly found the presence of optically clear nuclei (OCN) only in tumor cells of squamoid corpuscles. Recent studies showed that OCN seen in some types of neoplastic and non-neoplastic tissues were rich in biotin and revealed false-positive immunostaining by the avidin-biotin peroxidase complex method. We conducted an investigation to see if the same were true for pancreatoblastoma. As a result, the OCN were positively stained when the tissue section was incubated with peroxidase-labeled avidin or streptavidin and developed in a chromogen solution. The reaction was completely blocked by pretreatment with free avidin. An identical nuclear staining pattern was observed by the indirect immunoperoxidase method with the antibiotin antibody. Ultrastructurally, the OCN were occupied by filamentous substructures of chromatin measuring approximately 10 to 12 nm in diameter. These results suggested to us that the OCN in pancreatoblastomas were part of the unique nuclear change in which abundant biotin accumulated in association with the characteristically arranged chromatin substructure. When immunostaining with biotin as the linkage agent is performed in pancreatoblastomas, the false positivity in the OCN should be kept in mind to avoid any misinterpretation of the results.