Related Experiment Video
Updated: Jul 30, 2026
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
Published on: September 1, 2018
Gene transfer to cultured human endometrial stromal cells: a model to study cyclooxygenase-2 gene regulation
1Department of Obstetrics, Gynecology and Reproductive Sciences, University of Texas Medical School at Houston, 77030, USA. JHUANG@OBG.MED.UTH.TMC.EDU
Objective:
To compare and optimize conditions for gene transfer to human endometrial stromal cells derived from primary culture and to determine the effect of interleukin-1beta (IL-1beta) and phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator. on the promoter activity of the human cyclooxygenase-2 (COX-2) gene.
Design:
Prospective controlled study.
Setting:
Academic research laboratory.
Patient(S):
Women undergoing benign gynecologic surgery for indications other than endometrial diseases.
Intervention(S):
Endometrial stromal cells were used for transient transfection study.
Main Outcome Measure(S):
Luciferase activity in transfected endometrial stromal cells.
Result(S):
Gene transfer mediated by cationic lipid was more efficient and more consistent. Lipofectamine, a polycationic lipid, yielded the highest efficiency. Phorbol 12-myristate 13-acetate (30 nM) and IL-1beta (100 ng/mL) increased COX-2 promoter activity by 2.6-fold and 2.2-fold, respectively.
Conclusion(S):
Induction of COX-2 by IL-1beta and PMA suggests that COX-2 and prostaglandin have important roles in the growth and differentiation of endometrial stromal cells. This model can be used to explore the roles of different promoter regulatory elements in COX-2 gene activation.
