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Related Experiment Videos

Correlation between amniotic fluid optical density and L/S ratio

A J Sbarra, H Michlewitz, R J Selvaraj

    Obstetrics and Gynecology
    |November 1, 1976
    PubMed
    Summary
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    A new test simplifies L/S ratio determination. Amniotic fluid

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Obstetrics

    Background:

    • Lecithin/sphingomyelin (L/S) ratios are crucial for assessing fetal lung maturity.
    • Traditional L/S ratio determination is complex and time-consuming.

    Purpose of the Study:

    • To develop a simpler method for determining L/S ratios.
    • To establish a correlation between optical density and L/S ratios.

    Main Methods:

    • Amniotic fluid supernatants were collected.
    • Supernatants were centrifuged at 2000 g for 10 minutes.
    • Optical density was measured at 400 nm.

    Main Results:

    • Optical density measurements showed a correlation with L/S ratios.

    Related Experiment Videos

  • This method circumvents complex traditional L/S ratio determination.

    • Conclusions:

    • Optical density measurement offers a simplified approach to assessing fetal lung maturity.
    • This test provides a viable alternative to conventional L/S ratio analysis.