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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
Aiolos regulates B cell activation and maturation to effector state
J H Wang1, N Avitahl, A Cariappa
1Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown 02129, USA.
Immunity
|November 7, 1998
Summary
Aiolos deficiency in mice leads to B cell activation, auto-antibodies, and lymphomas. Specific B cell populations are significantly reduced, impacting immune responses.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Aiolos is a zinc finger DNA-binding protein highly expressed in mature B cells.
- Aiolos is homologous to the Ikaros protein, a known regulator of lymphocyte development.
Purpose of the Study:
- To investigate the role of Aiolos in B cell development and function.
- To characterize the immune phenotype of Aiolos-deficient mice.
Main Methods:
- Analysis of Aiolos-null mutant mice.
- Assessment of B cell surface phenotype and proliferative responses.
- In vivo evaluation of T cell-dependent B cell responses.
- Monitoring for auto-antibodies and B cell lymphomas.
Main Results:
- Aiolos-deficient peripheral B cells show an activated phenotype and augmented antigen receptor-mediated proliferation.
- Aiolos-null mice spontaneously develop germinal centers and elevated serum IgG and IgE without immunization.
- Aging Aiolos mutants frequently exhibit auto-antibodies and B cell lymphomas.
- Peritoneal, marginal zone, and recirculating bone marrow B cell populations are significantly reduced in Aiolos-deficient mice.
Conclusions:
- Aiolos plays a critical role in regulating B cell homeostasis and preventing autoimmunity.
- Loss of Aiolos function leads to B cell hyperactivity, spontaneous immune activation, and lymphomagenesis.
- Aiolos is essential for the maintenance of specific B cell subsets.
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