Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The RXRalpha ligand-dependent activation function 2 (AF-2) is important for mouse development

B Mascrez1, M Mark, A Dierich

  • 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS-INSERM-ULP-Collège de France, BP163, CU de Strasbourg, France.

Development (Cambridge, England)
|November 10, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex-specific efficacy and safety outcomes in patients with resectable stage III non-small-cell lung cancer (NSCLC) undergoing neoadjuvant therapies: a pooled analysis of the SAKK trials 16/96, 16/00, 16/01, 16/08 and 16/14.

ESMO open·2025
Same author

First Constraints on General Neutrino Interactions Based on KATRIN Data.

Physical review letters·2025
Same author

Neoadjuvant treatment does not influence PD-L1 expression in stage III non-small-cell lung cancer: a retrospective analysis of tumor samples from the trials SAKK 16/96, 16/00, 16/01, and 16/14.

ESMO open·2023
Same author

New Constraint on the Local Relic Neutrino Background Overdensity with the First KATRIN Data Runs.

Physical review letters·2022
Same author

Corrigendum to 'Long-term outcomes of operable stage III NSCLC in the pre-immunotherapy era: results from a pooled analysis of the SAKK 16/96, SAKK 16/00, SAKK 16/01, and SAKK 16/08 trials': [ESMO Open Volume 7, Issue 2, (2022), 100455].

ESMO open·2022
Same author

Long-term benefit of lurbinectedin as palliative chemotherapy in progressive malignant pleural mesothelioma (MPM): final efficacy and translational data of the SAKK 17/16 study.

ESMO open·2022
Same journal

Dissecting planar and vertical organiser signals in early chick neural development.

Development (Cambridge, England)·2026
Same journal

Real-time transcriptomic profiling of hPSC-derived cartilage during development identifies a key role for the extracellular matrix in homeostasis and protection.

Development (Cambridge, England)·2026
Same journal

In preprints - housekeeping the housekeeping genes.

Development (Cambridge, England)·2026
Same journal

In preprints - light, cluster, friction: a cell dance on the gastrulation stage.

Development (Cambridge, England)·2026
Same journal

PBX-dependent and -independent Hox programs establish and maintain motor neuron terminal identity.

Development (Cambridge, England)·2026
Same journal

NUDT21 regulates 3'UTR dynamics in epididymal principal cells to preserve sperm integrity.

Development (Cambridge, England)·2026
See all related articles

Disrupting the RXRalpha AF-2 core in mice causes developmental defects. These defects, mimicking vitamin A deficiency, highlight the critical role of RXRalpha

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Retinoid signaling is crucial for embryonic development, mediated by Retinoic Acid Receptors (RARs) and Retinoid X Receptors (RXRs).
  • The ligand-binding domain (LBD) of RXRalpha, particularly the Activation Function 2 (AF-2) core, is essential for its transcriptional activity.
  • Previous studies implicated RXRalpha in various developmental processes, but the precise role of its AF-2 domain remained unclear.

Purpose of the Study:

  • To investigate the functional importance of the RXRalpha AF-2 core in vivo.
  • To determine the contribution of RXRalpha AF-2 to retinoid signaling during embryonic development.
  • To elucidate the role of RXRalpha AF-2 in RAR/RXR heterodimer function.

Main Methods:

  • Generation of a mouse model with a specific deletion of the C-terminal 18 amino acids of RXRalpha, encompassing the AF-2 core.

Related Experiment Videos

  • Analysis of homozygous RXRalpha AF-2 mutants (RXRalpha af2(o)) and compound mutants with RXRbeta and/or RXRgamma deficiencies.
  • Comparative analysis of RXRalpha af2(o) compound mutants with RARs against known RXRalpha/RAR compound mutants and vitamin A deficiency models.

    • Main Results:

    • Homozygous RXRalpha af2(o) mutants exhibited partial developmental abnormalities, with lethality during late fetal development or at birth.
    • Compound mutants lacking RXRalpha AF-2 and RXRbeta (and/or RXRgamma) displayed severe malformations, closely resembling fetal vitamin A deficiency syndrome.
    • RX Ralpha af2(o)/RAR compound mutants showed defects similar to those observed in RXRalpha/RAR compound mutants, underscoring the AF-2 domain's role in heterodimer function.

    Conclusions:

    • The integrity of the RXRalpha AF-2 domain is vital for mediating developmental functions through RAR/RXR heterodimers.
    • Ligand-dependent transactivation by RXRalpha via its AF-2 domain is a critical component of retinoid signaling during embryogenesis.
    • This study highlights the essential role of RXRalpha's AF-2 domain in coordinating retinoid-mediated developmental processes.