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Related Experiment Videos

Eosinophil infiltration and degranulation in normal human tissue

M Kato1, G M Kephart, N J Talley

  • 1Department of Immunology and Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA.

The Anatomical Record
|November 12, 1998
PubMed
Summary
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Eosinophils infiltrate and degranulate in normal human tissues, primarily the gastrointestinal tract. Tissue procurement methods, like endoscopic forceps, can increase eosinophil degranulation in gut samples.

Area of Science:

  • Immunology
  • Gastroenterology

Background:

  • Eosinophil granule major basic protein (MBP) detection via immunofluorescence identifies eosinophil infiltration and degranulation.
  • Previous studies indicated eosinophil infiltration and degranulation in diseased human tissues and normal gut.
  • The current study expanded analysis to nearly all normal human organs.

Purpose of the Study:

  • To investigate eosinophil infiltration and degranulation across a comprehensive range of normal human tissues.
  • To determine if tissue procurement methods influence eosinophil degranulation in the normal gastrointestinal tract.

Main Methods:

  • Indirect immunofluorescence was used to detect MBP in 117 normal human biopsy/autopsy specimens from various organs.
  • Normal proximal jejunum samples from six patients were obtained using endoscopic forceps or a scalpel.

Related Experiment Videos

  • Immunofluorescence analysis compared eosinophil degranulation based on procurement method.

    • Main Results:

    • The gastrointestinal tract was the only normal organ exhibiting both significant eosinophil infiltration and degranulation.
    • Spleen, lymph node, and thymus showed eosinophil infiltration but minimal degranulation.
    • Eosinophil degranulation was significantly higher in jejunum specimens procured via endoscopic forceps compared to scalpel (P = 0.021).

    Conclusions:

    • Normal human organs consistently show both eosinophil infiltration and degranulation primarily in the gastrointestinal tract.
    • Tissue procurement techniques significantly impact the observed level of eosinophil degranulation in gut tissues.
    • Findings highlight the gut's unique eosinophil activity and the importance of standardized tissue handling.