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[An investigation on genotypes of hepatitis C virus in Anhui province]
Insights
Hepatitis C Virus (HCV) type II is the most common genotype in Anhui Province, China. HCV type II infections were more prevalent in southern regions, with significant geographical distribution differences observed.
Area of Science:
- Hepatology
- Virology
- Molecular Biology
Context:
- Hepatitis C Virus (HCV) poses a significant global health challenge.
- Understanding HCV genotype distribution is crucial for effective treatment and prevention strategies.
- Anhui Province, China, has a diverse population with varying risk factors for HCV infection.
Purpose:
- To investigate the distribution of HCV genotypes in Anhui Province.
- To identify the predominant HCV genotype and analyze its geographical prevalence.
- To explore potential correlations between HCV genotypes and patient demographics or transfusion history.
Summary:
- This study analyzed 371 hepatitis C patients, 60 haemodialysis patients, and 100 blood donors in Anhui Province.
- HCV RNA was detected using RT-PCR, and genotyping was performed via restriction fragment length polymorphism.
- HCV type II was the dominant genotype (75.66%), followed by type III (13.23%). HCV type II was more common in southern Anhui, with significant geographical variations (P < 0.05).
Impact:
- Identifies HCV type II as the predominant genotype in Anhui Province, guiding public health interventions.
- Highlights significant geographical variations in HCV genotype distribution, informing regional control efforts.
- Provides baseline data for future epidemiological studies and the development of targeted HCV management strategies in China.
Abstract:
To study the distribution of HCV genotypes in Anhui province, anti-HCV positive serum samples were collected from 371 hepatitis C patients, 60 patients under haemodialysis and 100 blood donors. HCVRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Based on the results of PCR positivity, HCV genotyping was investigated by restriction fragment length polymorphism. Results showed that among hepatitis C patients 143 (75.66%) were HCV type II infection, 25 (13.23%) were HCV type III and 12(6.35%) were type II/III co-infection. HCV type I and IV infection appeared in one of each (0.53%). HCV genotype could not be determined in 7 patients because of low HCVRNA titers. These results indicated that HCV II infection was the dominant genotype in Anhui Province. Geographically, HCV type II infection was higher in the southern areas. The patterns of genotype distribution in the southern and northern areas were significant different (P < 0.05). There was no significant difference between HCV genotypes in relation to the history of blood transfusion.