Simple determination of peroxyl radical-trapping capacity

A simple spectrophotometric method of determination of peroxyl radical-trapping capacity (PRTC) of body fluids and food products is proposed. In this method, decomposition of 2,2'-azobis(2-amidopropane) hydrochloride (ABAP) is the source of peroxyl and alkoxyl radicals which oxidize 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to a green cation radical. Antioxidant present in a sample inhibit the reaction; the induction time of the reaction is proposed as a parameter enabling determination of antioxidant content. Standard assay conditions are: 20 mM ABAP and 150 microM ABTS in 0.1 M phosphate buffer, pH 7.0, at 37 degrees C; absorbance is monitored at 414 nm. A 10-min assay allows for determination of the induction time of appropriately diluted sample. As examples of application of this method, PRTC values of several types of beverages are reported.