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Role of DNA definite structural elements in interaction with repair enzyme uracil-DNA glycosylase
E A Kubareva1, N L Vasilenko, O V Vorobjeva
1A. N. Belozersky Institute of Physical and Chemical Biology, Chemistry Department, Moscow State University, Russia. kubareva@genebee.msu.su
Abstract:
Interaction of different oligodeoxyribonucleotides (oligos), their analogs and oligonucleopeptide with uracil-DNA glycosylase (UDG) from human placenta was investigated. It is shown that there is no considerable contribution of heterocyclic bases of DNA to UDG-substrate binding but the UDG interaction with some DNA phosphate groups is necessary for enzyme-substrate recognition. However the phosphate group adjacent to single dU from the 3'-end in oligo is not involved into the electrostatic contact with UDG. It is found that UDG has the high affinity to its reaction product. An oligonucleotide containing a single 2'-deoxy-2'-aminouridine is a non-hydrolyzable substrate analog for UDG.