Augmentation in the expression of a ribosomal protein associated with transformation

Using differential hybridization technique, cDNA that was a partial sequence of rat ribosomal protein L41 was isolated from fos- and src-transfected rat fibroblast 3Y1. The cDNA was named #31. Subsequently, the human counterpart(named hm3168) was cloned from a human melanoma cell line(M14) and the amino acid sequence deduced from the cDNA proved to be identical to that of HG-12. The gene expression of hm3168 was inducible by serum-stimulation. It reveals the possible up-regulation of the transcription via fos-mediated signal transduction. Northern-blot and Western-blot analyses showed a large expression of the #31 gene in the rat transformed cell lines.