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Triple helix-forming oligonucleotides which make imperfect Watson-Crick duplexes that compete with the creation of
H Porumb1, H Gousset, E Taillandier
1Laboratoire de Spectroscopie Bio-moléculaire, Université Paris-Nord, Bobigny, France. porumb@infobiogen.fr
Electrophoresis
|November 20, 1998
Abstract:
Because of the repetitive nature of the sequence, when titrating a G,A-rich, triple helix-forming oligonucleotide (TFO) with increasing concentrations of target duplex in order to obtain the dissociation constant of the complex, a duplex is sometimes first generated at intermediate concentrations of the target. This duplex is based on an imperfect Watson-Crick pairing of the TFO to the pyrimidine-rich strand of the target. An explanation is proposed for this duplex being obtained only in a certain domain of the titration range, before the triple helix becomes predominant.