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Microorganisms in closed periapical lesions

M Abou-Rass1, G Bogen

  • 1Department of Endodontics, University of Southern California, School of Dentistry, Los Angeles 90089-0641, USA.

International Endodontic Journal
|November 21, 1998
PubMed
Summary
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Microorganisms, including obligate anaerobes, were found in periapical lesions resistant to endodontic therapy. These bacteria may prevent healing even after treatment.

Area of Science:

  • Microbiology
  • Endodontics
  • Oral Surgery

Background:

  • Periapical lesions often occur after endodontic treatment failure.
  • Pulpal calcification and refractory endodontic therapy can complicate lesion management.

Purpose of the Study:

  • To investigate the microbial composition of strictly selected closed periapical lesions.
  • To identify microorganisms associated with refractory endodontic therapy and pulpal calcification.

Main Methods:

  • Selected 13 periapical lesions from 70 patients based on strict criteria for clinical isolation.
  • Employed a controlled culturing method, taking samples from three sites during surgery (surgical window, lesion body, apex).

Main Results:

  • Microorganisms were consistently isolated from the apex of periapical lesions.

Related Experiment Videos

  • Obligate anaerobes (63.6%) and facultative anaerobes (36.4%) were identified.
  • Key species included Actinomyces sp., Propionibacterium sp., and Streptococcus sp.

    • Conclusions:

    • Closed periapical lesions in calcified or treatment-resistant teeth harbor bacteria.
    • Incomplete eradication of root canal microorganisms and anatomical factors may lead to persistent periapical infections and impede healing.