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Cells and cytokines in pollinosis
A G Carlos1, M L Carlos, M A Santos
1Clinica Universitaria de Medicina I, Instituto de Imunologia e Centro de Hematologia e Imunologia, Faculdade de Medicina de Lisboa, Portugal.
Allergie Et Immunologie
|November 21, 1998
Summary
Seasonal allergic rhinitis (pollinosis) involves immune cell changes. Pollen exposure decreases CD23 on B cells and shifts memory (CD45RO) and naive (CD45RA) cell populations, indicating cellular involvement in allergic responses.
Area of Science:
- Immunology
- Allergy Research
- Cellular Biology
Background:
- Pollinosis, or hay fever, serves as a natural model for studying self-limiting allergic diseases.
- Understanding the immune response during pollen exposure is crucial for managing allergic conditions.
Purpose of the Study:
- To investigate the dynamic changes in immune cell populations and soluble mediators during the pollen season.
- To identify cellular markers associated with allergic inflammation in pollinosis.
Main Methods:
- Flow cytometry was used to analyze various immune cell populations (CD2, CD4, CD8, CD19, CD22, CD23, CD28, CD29, CD45RA, CD45RO).
- Enzyme-linked immunosorbent assays (ELISA) measured soluble CD23 and cytokines (IL-2, IL-4, IL-2 soluble receptor).
- Samples were collected before, during, and after the pollen season.
Main Results:
- A decrease in CD23 on peripheral blood cells (PBC) was observed during the pollen season, persisting afterward.
- CD45RO memory cells decreased, while CD45RA naive cells increased during pollen exposure.
- Peripheral blood cells expressing CD29 showed reduced levels during peak pollen season.
Conclusions:
- Allergen exposure in pollinosis induces a turnover of CD45+ peripheral blood cells.
- A reduction in CD23 (low-affinity IgE receptor) on peripheral blood cells and consumption/binding of CD29+ cells occur.
- Cellular changes during pollen season can serve as potential biomarkers for allergic inflammation in pollinosis.