Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated amplified flow immunoassay for cocaine

C G Bauer1, A V Eremenko, A Kühn

  • 1Institute of Biochemistry and Molecular Physiology, University of Potsdam, Luckenwalde, Germany.

Analytical Chemistry
|November 21, 1998
PubMed
Summary

A novel amplified flow immunoassay (AFIA) enables rapid, picomolar detection of cocaine. This method combines immunoenzymometric assay with a biosensor for sensitive and efficient analysis of cocaine concentrations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"Optimal timing for RNA isolation: Recommendations for placenta sample collection under clinical conditions".

Placenta·2025
Same author

Kinematic Effects of Combined Subthalamic and Dorsolateral Nigral Deep Brain Stimulation in Parkinson's Disease.

Journal of Parkinson's disease·2024
Same author

Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds.

Nature nanotechnology·2023
Same author

Cell Type-Specific Decomposition of Gingival Tissue Transcriptomes.

Journal of dental research·2021
Same author

Pathogen-specific antibody profiles in patients with severe systemic infections.

European cells & materials·2020
Same author

[Erratum to: Diagnosis and treatment of interstitial cystitis (IC/PBS). S2k guideline of the German Society of Urology].

Der Urologe. Ausg. A·2019

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Biomedical Engineering

Background:

  • Hapten detection requires sensitive and rapid analytical methods.
  • Traditional immunoassays can lack the sensitivity or speed for certain applications.
  • Enzyme labels offer potential for signal amplification in immunoassays.

Purpose of the Study:

  • To develop and validate an amplified flow immunoassay (AFIA) for the sensitive detection of cocaine.
  • To integrate a noncompetitive immunoenzymometric assay (IEMA) with an on-line biosensor for real-time enzyme label detection.
  • To establish the analytical performance characteristics of the AFIA for cocaine analysis.

Main Methods:

  • Development of an AFIA combining IEMA with an alkaline phosphatase (ALP) label.

Related Experiment Videos

  • Utilized an affinity column with immobilized cocaine for separation of unbound antibody-label complexes.
  • Employed an amperometric substrate-recycling biosensor with tyrosinase and glucose dehydrogenase for ALP detection.

    • Main Results:

    • Achieved a lower limit of detection of 380 pM (38 fmol) for cocaine.
    • Demonstrated a sampling rate of 26 samples per hour.
    • Showed imprecision of +/- 10% and recovery of 49 +/- 3% in real samples.

    Conclusions:

    • AFIA is a viable approach for the rapid detection of picomolar concentrations of haptens like cocaine.
    • The integrated biosensor system provides sensitive and efficient on-line detection of enzyme labels.
    • This method offers a significant advancement in analytical techniques for small molecule detection.