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Microvascular damage secondary to thromboembologenic arterial injury--a new experimental model
M Thorwest1, J Fuglsang, J K Poulsen
1Institute of Experimental Clinical Research, Skejby Sygehus, Aarhus University Hospital, Arhus N, Denmark. thorwest@dadlnet.dk
Background And Aims:
The isolated rat cremaster model is used extensively for evaluating the microcirculation secondary to arterial injury. Current techniques, however, do not allow for assessment of injury and effect within the same animal. The purpose of this study was to develop a model incorporating the following points: visualization of the upstream arterial injury and the downstream microvascular damage in the same animal, analysis of capillary density by randomization of measuring windows throughout the cremaster muscle, and simplification of the arterial injury.
Materials And Methods:
Thirteen male Wistar rats were randomized into two groups. In group I, the entire isolation of the cremaster muscle was performed, without arterial damage. In group II, arterial damage consisting of a standardized pinch was applied to the feeding vessel.
Results And Conclusions:
It was possible to produce a simplified arterial injury and visualize the resulting downstream microvascular damage in the same animal, in a quantitative and randomized fashion. In group I, no thrombus formation was seen. In group II, all animals produced an embolizing arterial thrombus, which was dynamic within the first hour of observation. Capillary density was reduced from 6.5 to 3.5 capillaries/measuring window within the first hour after arterial thromboembolism.