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Related Experiment Videos

SV40-Mediated immortalization

K K Jha1, S Banga, V Palejwala

  • 1Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, New Jersey, 07103-2714, USA.

Experimental Cell Research
|November 26, 1998
PubMed
Summary
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Replicative senescence limits human cell lifespan, acting as a tumor suppression mechanism. Introducing SV40 sequences revealed multiple facets of cell immortalization and identified a novel growth suppressor gene, SEN6.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Oncology

Background:

  • Human diploid cells undergo replicative senescence, limiting their lifespan.
  • Tumor-derived cell lines are immortal, suggesting senescence is a tumor suppression mechanism.
  • Understanding cell immortalization is crucial for cancer research.

Purpose of the Study:

  • To dissect the mechanisms of cell immortalization.
  • To develop matched sets of nonimmortal and immortal cell lines using SV40 sequences.
  • To identify novel genes involved in growth suppression and cellular aging.

Main Methods:

  • Utilizing Simian virus 40 (SV40) sequences to induce immortalization in human diploid cells.
  • Developing matched pairs of nonimmortal and immortal cell lines for comparative analysis.

Related Experiment Videos

  • Investigating both SV40-dependent and -independent pathways of immortalization.

    • Main Results:

    • Cell immortalization involves multiple facets, including SV40-dependent and -independent aspects.
    • Identification of a novel growth suppressor gene, SEN6.
    • Establishment of a model system for studying cellular aging, apoptosis, and telomere stabilization.

    Conclusions:

    • Replicative senescence is a critical tumor suppression mechanism.
    • SV40-transformed cells provide a valuable model for studying oncogene and growth suppressor gene alterations.
    • Further research using this model system can yield insights into cellular aging and cancer development.