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Related Experiment Videos

Increased MAP1B expression without increased phosphorylation in manganese-treated PC12Mn cells

K E Larsen1, M Pacheco, J Roth

  • 1Department of Pharmacology and Toxicology, University at Buffalo School of Medicine and Biomedical Sciences, Buffalo, New York, 14214-3000, USA.

Experimental Cell Research
|November 26, 1998
PubMed
Summary

Manganese chloride stimulates neurite outgrowth in PC12 cells by increasing Microtubule-Associated Protein 1B (MAP1B) expression, not its phosphorylation. Early neurite growth can occur without elevated MAP1B phosphorylation.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Microtubule-Associated Protein 1B (MAP1B) regulation is crucial for neuronal development and axon growth.
  • PC12 cell variants offer a model for studying early neurite outgrowth.
  • Understanding MAP1B's role in neuritogenesis is key to neuronal development research.

Purpose of the Study:

  • To investigate the effect of manganese chloride on MAP1B expression and phosphorylation in a novel PC12 cell variant.
  • To determine if MAP1B phosphorylation is a prerequisite for early neurite outgrowth.
  • To elucidate the distinct roles of MAP1B expression and phosphorylation in neuronal regeneration.

Main Methods:

  • Utilized a novel PC12 cell variant exhibiting neurite outgrowth features upon manganese chloride stimulation.

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  • Assessed MAP1B protein levels via immunoblots.
  • Measured MAP1B phosphorylation using [32P]orthophosphate metabolic radiolabeling and a phospho-specific antibody (SMI 31).

    • Main Results:

    • Manganese chloride significantly increased MAP1B protein expression within 12 to 24 hours.
    • No significant change in MAP1B phosphorylation was observed during manganese-induced early neurite outgrowth.
    • Manganese induced transient neurite regeneration in NGF-primed PC12 cells, with sustained regeneration showing increased MAP1B phosphorylation.

    Conclusions:

    • Early stages of neurite outgrowth, including cell spreading and short extension formation, do not necessitate increased MAP1B phosphorylation.
    • MAP1B expression, rather than its phosphorylation, is a key factor in the initial phase of manganese-induced neurite outgrowth.
    • Distinct mechanisms regulate MAP1B expression and phosphorylation during different phases of neuronal regeneration.