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Determination of eprinomectin in plasma by high-performance liquid chromatography with automated solid phase
J F Sutra1, C Chartier, P Galtier
1Laboratoire de Pharmacologie-Toxicologie, INRA, Toulouse, France.
Abstract:
A method is described for the determination of eprinomectin in plasma using high-performance liquid chromatography with fluorescence detection (excitation and emission wavelengths 355 and 465 nm, respectively). The fluorescent derivative was obtained by a condensation reaction with trifluoroacetic anhydride and N-methylimidazole. The method employs 1 ml plasma samples and gives linear calibration graphs (r = 0.999) over the concentration range studied (0.5-50 ng ml-1). Solid extraction using the benchmate procedure was used for sample preparation. This method permits the determination of eprinomectin at levels as low as 0.1 ng ml-1 and is suitable for the pharmacokinetic study of eprinomectin in animals.