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Effects of sample preparation conditions on biomolecular solid-state NMR lineshapes
D L Jakeman1, D J Mitchell, W A Shuttleworth
1Department of Biochemistry and Biophysics, Washington State University, Pullman 99163-4660, USA.
Journal of Biomolecular NMR
|December 3, 1998
Abstract:
Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of 31P resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 +/- 12 Hz and 125 +/- 7 Hz respectively, by a variety of methods involving additives and freezing techniques.