Related Experiment Video

Updated: Apr 20, 2026

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Published on: August 19, 2021

Kinetic evaluation of glucose 1-phosphate analogues with a thymidylyltransferase using a continuous coupled enzyme

S M Forget¹, A Jee, D A Smithen

¹Department of Chemistry, Dalhousie University, 6274 Coburg Rd, PO Box 15, 000, Halifax, Nova Scotia B3H 4R2, Canada. David.Jakeman@dal.ca.

Organic & Biomolecular Chemistry

|November 20, 2014

Abstract:

Cps2L, a thymidylytransferase, is the first enzyme in Streptococcus pneumoniae L-rhamnose biosynthesis and an antibacterial target. We herein report the evaluation of six sugar phosphate analogues selected to further probe Cps2L substrate tolerance. A modified continuous spectrophotometric assay was employed for facile detection of pyrophosphate (PPi) released from nucleotidylyltransfase-catalysed condensation of sugar 1-phosphates and nucleoside triphosphates to produce sugar nucleotides. Additionally, experiments using waterLOGSY NMR spectroscopy were investigated as a complimentary method to evaluate binding affinity to Cps2L.

More Related Videos

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

Measuring In Vitro ATPase Activity for Enzymatic Characterization

Measuring In Vitro ATPase Activity for Enzymatic Characterization

Published on: August 23, 2016

Related Concept Videos

Glycolysis: Preparatory Phase

Glycolysis: Preparatory Phase

In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...

ATP Energy Storage and Release

ATP Energy Storage and Release

ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...

Articles linked to this work by shared authors, journal, and citation graph.

[Inactivation of Ras1 in Fission Yeast Aggravates the Oxidative Stress Response Induced by Tert Butyl Hydroperoxide (tBHP)].

Molekuliarnaia biologiia·2023

38. Effect of Chlorhexidine Rinsed Blood Contamination on Bond Strength of Brackets Bonded with Self-etch Primer- An in Vitro Study.

Mymensingh medical journal : MMJ·2023

Retraction notice to "Synergistic effects of thymoquinone and curcumin on immune response and anti-viral activity against avian influenza virus (H9N2) in turkeys" [Poult. Sci. 95 (2016) 1513-1520].

Poultry science·2020

Synergic effects of cancer stem cells markers, CD44 and embryonic stem cell transcription factor Nanog, on bladder cancer prognosis.

British journal of biomedical science·2019

A possible direct action of oxytocin on spermatogenesis and steroidogenesis in pre-pubertal mouse.

Andrologia·2018

Biosynthetic 4,6-dehydratase gene deletion: isolation of a glucosylated jadomycin natural product provides insight into the substrate specificity of glycosyltransferase JadS.

Organic & biomolecular chemistry·2017

See all related articles