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Decrease of the obese gene expression in bovine subcutaneous adipose tissue by fasting
1Department of Animal Science, Faculty of Agriculture, Utsunomiya University, Japan.
Bioscience, Biotechnology, and Biochemistry
|December 4, 1998
Summary
Food deprivation significantly decreased leptin mRNA levels in bovine adipose tissue. Refeeding partially restored obese gene expression, indicating feeding regulates this in ruminants.
Area of Science:
- Animal Science
- Molecular Biology
- Genetics
Background:
- Leptin, a product of the obese gene, plays a crucial role in regulating energy balance and metabolism.
- Understanding leptin gene expression in livestock is vital for optimizing animal health and productivity.
Purpose of the Study:
- To investigate the regulation of leptin (obese gene) mRNA expression in bovine adipose tissue.
- To determine the effect of feeding status on obese gene expression in ruminants.
Main Methods:
- Analysis of leptin mRNA expression using lysate RNase protection assay in bovine adipose tissue.
- Experimental manipulation involving two days of food deprivation followed by three hours of refeeding.
Main Results:
- A significant decrease in leptin mRNA levels was observed following two days of food deprivation.
- Partial recovery of obese gene expression was noted after a brief three-hour refeeding period.
Conclusions:
- Bovine obese gene expression in adipose tissue is significantly influenced by nutritional status.
- Feeding acts as a regulatory mechanism for obese gene expression in ruminants, impacting leptin levels.