Effects of acute normovolemic hemodilution on T2*-weighted images of rat brain

Acute normovolemic hemodilution (HD) was induced in anesthetized rats to assess the effect of changes in hematocrit (Hct) on signal intensity in T2*-weighted magnetic resonance (MR) images. Other relevant physiological parameters were maintained invariant. Two degrees of HD were induced: mild (Hct reduced from 42.6+/-2.2% to 33.4+/-2.1%) and moderate (Hct reduced from 44.6+/-2.7% to 26.2+/-1.7%). A two-dimensional gradient-echo sequence was used to monitor signal changes with high temporal resolution before, during, and after HD protocols. The time course of signal intensity change was closely related to that of changes in Hct. Corresponding changes in R2* (deltaR2*) with respect to the pre-HD state were calculated for the brain parenchyma. Average deltaR2* values of -0.24+/-0.06 s(-1) and -0.40+/-0.07 s(-1) were obtained for the mild and moderate HD groups, respectively, during the final 2 min of MR imaging (proximal to correlative measurements of Hct). MR measured deltaR2* values were in close agreement with the expected changes in R2* predicted from theory when the measured changes in Hct were used as independent variables. These data are in good agreement with the current understanding of the effects of changes in the intravascular concentration of deoxyhemoglobin on induced magnetic susceptibility and hold promise for quantitative measurement of brain oxygenation in vivo.