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Related Experiment Videos

B cells directly tolerize CD8(+) T cells

S R Bennett1, F R Carbone, T Toy

  • 1Immunology Division, The Walter and Eliza Hall Institute of Medical Research, The Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia.

The Journal of Experimental Medicine
|December 8, 1998
PubMed
Summary
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B cells can directly induce tolerance in CD8(+) T cells. This process involves antigen presentation by B cells, leading to T cell death via Fas (CD95) signaling, demonstrating a novel immune tolerance mechanism.

Area of Science:

  • Immunology
  • Cellular immunology
  • T cell biology

Background:

  • Cytotoxic T lymphocytes (CTLs), specifically CD8(+) T cells, play a crucial role in adaptive immunity.
  • Antigen presentation by B cells is critical for T cell activation and immune responses.
  • Mechanisms of T cell tolerance are essential for preventing autoimmunity.

Purpose of the Study:

  • To investigate the direct impact of B cells presenting antigens on CD8(+) T cell responses.
  • To elucidate the mechanism by which B cells induce tolerance in CD8(+) T cells.
  • To determine the role of Fas (CD95) signaling in B cell-mediated T cell tolerance.

Main Methods:

  • In vitro co-culture of antigen-specific CD8(+) T cells (OT-I cells) with antigen-bearing B cells.
  • Assessment of OT-I T cell survival and B cell killing upon antigen recognition.

Related Experiment Videos

  • In vivo adoptive transfer experiments using OT-I T cells in mice coinjected with antigen-bearing B cells.
  • Utilized OT-I T cells deficient in CD95 (Fas) to investigate the role of this molecule.

    • Main Results:

    • Antigen recognition by OT-I T cells led to B cell killing and significant OT-I T cell death.
    • T cell death was mediated by Fas (CD95) signaling, as CD95-deficient OT-I cells showed improved survival.
    • In vivo, adoptively transferred OT-I T cells proliferated transiently and were subsequently lost from lymphoid compartments after encountering antigen-bearing B cells.

    Conclusions:

    • B cells can directly induce tolerance in CD8(+) T cells, a previously undescribed phenomenon.
    • The primary mechanism of this B cell-mediated tolerance involves activation-induced cell death (AICD) of CD8(+) T cells.
    • Fas (CD95) signaling is a key mediator of this B cell-induced CD8(+) T cell deletion.