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Thyroxine binding globulin (TBG) assay by radial immunodiffusion
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|July 1, 1978
Summary
Thyroxine-binding globulin (TBG) levels were measured using radial immunodiffusion. This method showed good agreement with existing thyroxine-binding capacity tests, validating its use for assessing TBG levels.
Area of Science:
- Endocrinology
- Clinical Chemistry
- Immunology
Background:
- Thyroxine-binding globulin (TBG) is crucial for thyroid hormone transport.
- Accurate measurement of TBG is essential for diagnosing thyroid disorders.
Purpose of the Study:
- To evaluate the efficacy of radial immunodiffusion for quantifying serum TBG.
- To compare TBG measurements with established methods for thyroxine-binding capacity.
Main Methods:
- Serum samples were analyzed using radial immunodiffusion.
- A small volume of ten microliters of serum was utilized.
- Results were correlated with thyroxine-binding capacity determined by paper electrophoresis.
Main Results:
- The mean TBG level in a normal cohort was 1.95 +/- 0.38 mg/dl.
- A high correlation (r = 0.956) was observed between radial immunodiffusion and paper electrophoresis.
- The method demonstrated good agreement with the established technique.
Conclusions:
- Radial immunodiffusion is a reliable method for measuring thyroxine-binding globulin.
- This technique offers a valid alternative for assessing TBG levels in clinical settings.
- The findings support the use of radial immunodiffusion in routine diagnostics.